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Sedimentation equilibrium
Sedimentation equilibrium in a solution or suspension of different particles, such as molecules, exists when the rate of transport of each material in any one direction due to sedimentation equals the rate of transport in the opposite direction due to diffusion. Sedimentation is due to an external force, such as gravity (for very large particles) or centrifugal force in a centrifuge.
Modern applications use the analytical ultracentrifuge. The theoretical basis for the measurements is developed from the Mason-Weaver equation. The advantage of using analytical sedimentation equilibrium analysis for Molecular Weight of proteins and their interacting mixtures is the avoidance of need for derivation of a frictional coefficient, otherwise required for interpretation of dynamic sedimentation.
It was discovered for large particles by Jean Baptiste Perrin for which he received the Nobel Prize in Physics in 1926.
==Application in determining molecular mass==
Sedimentation equilibrium can be used to determine molecular mass. It forms the basis for an analytical ultracentrifugation method for measuring molecular masses, such as those of proteins, in solution.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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